What is it about?
Loop mediated isothermal amplification (LAMP) amplifies DNA and makes different sized products. We created a method, in the form of a mathematical model, where a researcher can predict the size of these different sized products based on the length of the primers they use. This prediction can be compared with LAMP results on gel electrophoresis to determine if the target was amplified specifically.
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Why is it important?
Current methods for testing for specific amplification for LAMP include noting a banding pattern during gel electrophoresis or performing a restriction enzyme digestion. This approach, though, will allow researchers to predict what a specific LAMP reaction will look like with electrophoresis deteciton before performing the amplification and allows for simple validation as soon as the assay is completed.
Perspectives
This mathematical model is important because as LAMP is used more widely, it is essential that there are quick, simple tools to validate specific reactions. Reaction specificity is always a key factor in any diagnosis so this tool contributes a simple method for testing specificity of a reaction quickly.
Lindsay Schneider
Brown University
Read the Original
This page is a summary of: Mathematical model to reduce loop mediated isothermal amplification (LAMP) false‐positive diagnosis, Electrophoresis, June 2019, Wiley,
DOI: 10.1002/elps.201900167.
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