All Stories

  1. Transcriptomic Analysis Reveals That Excessive Thyroid Hormone Signaling Impairs Phototransduction and Mitochondrial Bioenergetics and Induces Cellular Stress in Mouse Cone Photoreceptors

    Article • International Journal of Molecular Sciences, July 2024, MDPI AG

    Dr Willard M Freeman

  2. Heterochronic Plasma Transfer: Experimental Design, Considerations, and Technical Challenges

    Article • Rejuvenation Research, October 2023, Mary Ann Liebert Inc

    Dr Willard M Freeman

  3. Microglial senescence contributes to female-biased neuroinflammation in the aging mouse hippocampus: implications for Alzheimer’s disease

    Article • Journal of Neuroinflammation, August 2023, Springer Science + Business Media

    Dr Willard M Freeman

  4. Weight Loss Results in Increased Expression of Anti‐Inflammatory Protein CRISPLD2 in Mouse Adipose Tissue

    Article • Obesity, November 2019, Wiley

    Dr Willard M Freeman

  5. Early-life DNA methylation profiles are indicative of age-related transcriptome changes

    Article • Epigenetics & Chromatin, October 2019, Springer Science + Business Media

    Dr Willard M Freeman

  6. Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies

    Article • GeroScience, September 2019, Springer Science + Business Media

    Dr Willard M Freeman

  7. Short-term Calorie Restriction and 17α-Estradiol Administration Elicit Divergent Effects on Proteostatic Processes and Protein Content in Metabolically Active Tissues

    Article • The Journals of Gerontology Series A, May 2019, Oxford University Press (OUP)

    Dr Willard M Freeman

  8. Mitochondrial oxidative stress impairs contractile function but paradoxically increases muscle mass via fibre branching

    Article • Journal of Cachexia Sarcopenia and Muscle, February 2019, Wiley

    Dr Willard M Freeman

  9. Revisiting the genomic hypomethylation hypothesis of aging

    Article • Annals of the New York Academy of Sciences, January 2018, Wiley

    Dr Willard M Freeman

  10. Analysis of DNA modifications in aging research

    Article • GeroScience, January 2018, Springer Science + Business Media

    Dr Willard M Freeman

  11. 17α-estradiol acts through hypothalamic pro-opiomelanocortin expressing neurons to reduce feeding behavior

    Article • Aging Cell, November 2017, Wiley

    Dr Willard M Freeman

  12. Functional changes in the neural retina occur in the absence of mitochondrial dysfunction in a rodent model of diabetic retinopathy

    Article • Journal of Neurochemistry, October 2017, Wiley

    Dr Willard M Freeman

  13. Sexually divergent DNA methylation patterns with hippocampal aging

    Article • Aging Cell, September 2017, Wiley

    Dr Willard M Freeman

  14. Sexually divergent induction of microglial-associated neuroinflammation with hippocampal aging

    Article • Journal of Neuroinflammation, July 2017, Springer Science + Business Media

    Dr Willard M Freeman

  15. Sexually divergent DNA methylation programs with hippocampal aging

    Article • July 2017, Cold Spring Harbor Laboratory Press

    Dr Willard M Freeman

  16. Role of DNA methylation in the dietary restriction mediated cellular memory

    Article • GeroScience, May 2017, Springer Science + Business Media

    Dr Willard M Freeman

  17. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation

    Article • January 2017, Springer Science + Business Media

    Dr Willard M Freeman

  18. Loss of the antioxidant enzyme CuZnSOD (Sod1) mimics an age-related increase in absolute mitochondrial DNA copy number in the skeletal muscle

    Article • AGE, July 2016, Springer Science + Business Media

    Dr Willard M Freeman

  19. Absence of genomic hypomethylation or regulation of cytosine-modifying enzymes with aging in male and female mice

    Article • Epigenetics & Chromatin, July 2016, Springer Science + Business Media

    Dr Willard M Freeman

  20. Assessment of individual differences in the rat nucleus accumbens transcriptome following taste-heroin extended access

    Article • Brain Research Bulletin, May 2016, Elsevier

    Dr Willard M Freeman

  21. Reward devaluation and heroin escalation is associated with differential expression of CRF signaling genes

    Article • Brain Research Bulletin, May 2016, Elsevier

    Dr Willard M Freeman

  22. Bisulfite oligonucleotide-capture sequencing for targeted base- and strand-specific absolute 5-methylcytosine quantitation

    Article • AGE, April 2016, Springer Science + Business Media

    Dr Willard M Freeman

  23. CNS-wide Sexually Dimorphic Induction of the Major Histocompatibility Complex 1 Pathway With Aging

    Article • The Journals of Gerontology Series A, January 2016, Oxford University Press (OUP)

    Dr Willard M Freeman

  24. Detrimental effects of duplicate reads and low complexity regions on RNA- and ChIP-seq data

    Article • BMC Bioinformatics, December 2015, Springer Science + Business Media

    Dr Willard M Freeman

  25. Recent Developments in Understanding Brain Aging: Implications for Alzheimer’s Disease and Vascular Cognitive Impairment

    Article • The Journals of Gerontology Series A, November 2015, Oxford University Press (OUP)

    Dr Willard M Freeman

  26. Targeted DNA Methylation Analysis by Next-generation Sequencing

    Article • Journal of Visualized Experiments, February 2015, MyJove Corporation

    Dr Willard M Freeman

  27. Insulin treatment normalizes retinal neuroinflammation but not markers of synapse loss in diabetic rats

    Article • Experimental Eye Research, August 2014, Elsevier

    Dr Willard M Freeman

  28. Hippocampal Subregions Exhibit Both Distinct and Shared Transcriptomic Responses to Aging and Nonneurodegenerative Cognitive Decline

    Article • The Journals of Gerontology Series A, July 2014, Oxford University Press (OUP)

    Dr Willard M Freeman

  29. Integrative transcriptomic and proteomic analysis of osteocytic cells exposed to fluid flow reveals novel mechano-sensitive signaling pathways

    Article • Journal of Biomechanics, June 2014, Elsevier

    Dr Willard M Freeman

  30. Effect of cold perfusion and perfluorocarbons on liver graft ischemia in a donation after cardiac death model

    Article • Journal of Surgical Research, May 2014, Elsevier

    Dr Willard M Freeman

  31. Expression of NgR1-Antagonizing Proteins Decreases with Aging and Cognitive Decline in Rat Hippocampus

    Article • Cellular and Molecular Neurobiology, March 2013, Springer Science + Business Media

    William sonntag, Dr Willard M Freeman

  32. Increased hippocampal NgR1 signaling machinery in aged rats with deficits of spatial cognition

    Article • European Journal of Neuroscience, February 2013, Wiley

    William sonntag, Dr Willard M Freeman

  33. Focused, high accuracy 5-methylcytosine quantitation with base resolution by benchtop next-generation sequencing

    Article • Epigenetics & Chromatin, January 2013, Springer Science + Business Media

    Dr Willard M Freeman

  34. Nanoliposomal minocycline for ocular drug delivery

    Article • Nanomedicine Nanotechnology Biology and Medicine, January 2013, Elsevier

    Dr Willard M Freeman

  35. Protein biomarkers of alcohol abuse

    Article • Expert Review of Proteomics, August 2012, Taylor & Francis

    Dr Willard M Freeman

  36. Differential Gene Expression in Tamoxifen-Resistant Breast Cancer Cells Revealed by a New Analytical Model of RNA-Seq Data

    Article • PLoS ONE, July 2012, PLOS

    Dr Willard M Freeman

  37. Quantification of Hepatic UDP Glucuronosyltransferase 1A Splice Variant Expression and Correlation of UDP Glucuronosyltransferase 1A1 Variant Expression with Glucuronidation Activity

    Article • Journal of Pharmacology and Experimental Therapeutics, June 2012, American Society for Pharmacology & Experimental Therapeutics (ASPET)

    Dr Willard M Freeman

  38. Neuroglial Expression of the MHCI Pathway and PirB Receptor Is Upregulated in the Hippocampus with Advanced Aging

    Article • Journal of Molecular Neuroscience, May 2012, Springer Science + Business Media

    Dr Willard M Freeman

  39. Hippocampal expression of myelin-associated inhibitors is induced with age-related cognitive decline and correlates with deficits of spatial learning and memory

    Article • Journal of Neurochemistry, February 2012, Wiley

    Dr Willard M Freeman

  40. The Kinetics of Cardiopulmonary Bypass: A Dual-Platform Proteomics Study of Plasma Biomarkers in Pediatric Patients Undergoing Cardiopulmonary Bypass

    Article • Artificial Organs, January 2012, Wiley

    Dr Willard M Freeman

  41. A Longitudinal Analysis of Circulating Stress-Related Proteins and Chronic Ethanol Self-Administration in Cynomolgus Macaques

    Article • Alcoholism Clinical and Experimental Research, December 2011, Wiley

    Dr Willard M Freeman

  42. Plasma proteomic alterations in non-human primates and humans after chronic alcohol self-administration

    Article • The International Journal of Neuropsychopharmacology, August 2011, Oxford University Press (OUP)

    Dr Willard M Freeman

  43. Circulating IGF1 regulates hippocampal IGF1 levels and brain gene expression during adolescence

    Article • Journal of Endocrinology, July 2011, Bioscientifica

    Dr Willard M Freeman

  44. Hippocampal dysregulation of synaptic plasticity-associated proteins with age-related cognitive decline

    Article • Neurobiology of Disease, July 2011, Elsevier

    Dr Willard M Freeman

  45. Chronic insulin treatment of diabetes does not fully normalize alterations in the retinal transcriptome

    Article • BMC Medical Genomics, May 2011, Springer Science + Business Media

    Dr Willard M Freeman

  46. TPH2 in the ventral tegmental area of the male rat brain

    Article • Brain Research Bulletin, April 2011, Elsevier

    Dr Willard M Freeman

  47. Multi-Modal Proteomic Analysis of Retinal Protein Expression Alterations in a Rat Model of Diabetic Retinopathy

    Article • PLoS ONE, January 2011, PLOS

    Dr Willard M Freeman

  48. Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment

    Article • Journal of Neuroinflammation, January 2011, Springer Science + Business Media

    Dr Willard M Freeman

  49. The Hippocampal Neuroproteome with Aging and Cognitive Decline: Past Progress and Future Directions

    Article • Frontiers in Aging Neuroscience, January 2011, Frontiers

    Dr Willard M Freeman

  50. Individual Differences in Hyperlipidemia and Vitamin E Status in Response to Chronic Alcohol Self-Administration in Cynomolgus Monkeys

    Article • Alcoholism Clinical and Experimental Research, November 2010, Wiley

    Dr Willard M Freeman

  51. Effects of Ischemic Preconditioning and Bevacizumab on Apoptosis and Vascular Permeability Following Retinal Ischemia–Reperfusion Injury

    Article • Investigative Ophthalmology & Visual Science, November 2010, Association for Research in Vision and Ophthalmology (ARVO)

    Dr Willard M Freeman

  52. Phosphorimager

    Article • September 2010, Wiley

    Dr Willard M Freeman

  53. Classification of Alcohol Abuse by Plasma Protein Biomarkers

    Article • Biological Psychiatry, August 2010, Elsevier

    Dr Willard M Freeman

  54. Dual-Platform Proteomics Study of Plasma Biomarkers in Pediatric Patients Undergoing Cardiopulmonary Bypass

    Article • Pediatric Research, June 2010, Nature

    Dr Willard M Freeman

  55. Future Prospects for Biomarkers of Alcohol Consumption and Alcohol-Induced Disorders

    Article • Alcoholism Clinical and Experimental Research, April 2010, Wiley

    Dr Willard M Freeman

  56. Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome

    Article • Journal of Neurochemistry, March 2010, Wiley

    Dr Willard M Freeman

  57. The use of neuroproteomics in drug abuse research

    Article • Drug and Alcohol Dependence, February 2010, Elsevier

    Dr Willard M Freeman

  58. Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization

    Article • Proteome Science, January 2010, Springer Science + Business Media

    Dr Willard M Freeman

  59. Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice

    Article • Proteome Science, January 2010, Springer Science + Business Media

    Dr Willard M Freeman

  60. FOXO3a elicits a pro-apoptotic transcription program and cellular response to human lung carcinogen nicotine-derived nitrosaminoketone (NNK)

    Article • Lung Cancer, January 2010, Elsevier

    Dr Willard M Freeman

  61. Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration

    Article • BMC Neuroscience, January 2010, Springer Science + Business Media

    Dr Willard M Freeman, Drake Morgan

  62. Circulating Cytokines as Biomarkers of Alcohol Abuse and Alcoholism

    Article • Journal of Neuroimmune Pharmacology, December 2009, Springer Science + Business Media

    Dr Willard M Freeman

  63. A multistep validation process of biomarkers for preclinical drug development

    Article • The Pharmacogenomics Journal, December 2009, Nature

    Dr Willard M Freeman

  64. Transcriptomic comparison of the retina in two mouse models of diabetes

    Article • Journal of Ocular Biology Diseases and Informatics, December 2009, Springer Science + Business Media

    Dr Willard M Freeman

  65. Penn State Hershey—Center for Pediatric Cardiovascular Research

    Article • Artificial Organs, November 2009, Wiley

    Dr Willard M Freeman

  66. Improved membrane protein solubilization and clean-up for optimum two-dimensional electrophoresis utilizing GLUT-1 as a classic integral membrane protein

    Article • Journal of Neuroscience Methods, October 2009, Elsevier

    Dr Willard M Freeman

  67. Down-Regulation of 14-3-3 Isoforms and Annexin A5 Proteins in Lung Adenocarcinoma Induced by the Tobacco-Specific Nitrosamine NNK in the A/J Mouse Revealed by Proteomic Analysis

    Article • Journal of Proteome Research, August 2009, American Chemical Society (ACS)

    Dr Willard M Freeman

  68. Human Embryonic and Mesenchymal Stem Cells Express Different Nuclear Proteomes

    Article • Stem Cells and Development, June 2009, Mary Ann Liebert Inc

    Dr Willard M Freeman

  69. 2-D DIGE identification of differentially expressed heterogeneous nuclear ribonucleoproteins and transcription factors during neural differentiation of human embryonic stem cells

    Article • PROTEOMICS - CLINICAL APPLICATIONS, April 2009, Wiley

    Dr Willard M Freeman

  70. Persistent proteomic alterations in the medial prefrontal cortex with abstinence from cocaine self-administration

    Article • PROTEOMICS - CLINICAL APPLICATIONS, April 2009, Wiley

    Dr Willard M Freeman, Drake Morgan

  71. Cognitive performance and age-related changes in the hippocampal proteome

    Article • Neuroscience, March 2009, Elsevier

    Dr Willard M Freeman

  72. Gene expression profiles in HPV-immortalized human cervical cells treated with the nicotine-derived carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone

    Article • Chemico-Biological Interactions, February 2009, Elsevier

    Dr Willard M Freeman

  73. Gene expression changes following extinction testing in a heroin behavioral incubation model

    Article • BMC Neuroscience, January 2009, Springer Science + Business Media

    Dr Willard M Freeman

  74. The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome

    Article • Proteome Science, January 2009, Springer Science + Business Media

    Dr Willard M Freeman

  75. The Retinal Proteome in Experimental Diabetic Retinopathy

    Article • Molecular & Cellular Proteomics, December 2008, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Willard M Freeman

  76. A CSF biomarker panel for identification of patients with amyotrophic lateral sclerosis

    Article • Neurology, November 2008, Wolters Kluwer Health

    Dr Willard M Freeman

  77. Age-related changes in the expression and oxidation of bronchoalveolar lavage proteins in the rat

    Article • AJP Lung Cellular and Molecular Physiology, October 2008, American Physiological Society

    Dr Willard M Freeman

  78. Correlating Human and Animal Studies of Cocaine Abuse and Gene Expression

    Article • Annals of the New York Academy of Sciences, October 2008, Wiley

    Dr Willard M Freeman

  79. Heroin self-administration: II. CNS gene expression following withdrawal and cue-induced drug-seeking behavior

    Article • Pharmacology Biochemistry and Behavior, September 2008, Elsevier

    Dr Willard M Freeman

  80. Diabetes downregulates presynaptic proteins and reduces basal synapsin I phosphorylation in rat retina

    Article • European Journal of Neuroscience, July 2008, Wiley

    Dr Willard M Freeman

  81. Plasma Biomarkers in Pediatric Patients Undergoing Cardiopulmonary Bypass

    Article • Pediatric Research, June 2008, Nature

    Dr Willard M Freeman

  82. Twenty-five years of quantitative PCR for gene expression analysis

    Article • BioTechniques, April 2008, Future Science

    Dr Willard M Freeman

  83. CNS Genes Implicated in Relapse

    Article • Substance Abuse Research and Treatment, January 2008, SAGE Publications

    Dr Willard M Freeman

  84. Persistent Alterations in Mesolimbic Gene Expression with Abstinence from Cocaine Self-Administration

    Article • Neuropsychopharmacology, September 2007, Nature

    Dr Willard M Freeman, Drake Morgan

  85. A threshold neurotoxic amphetamine exposure inhibits parietal cortex expression of synaptic plasticity-related genes

    Article • Neuroscience, January 2007, Elsevier

    Dr Willard M Freeman

  86. Diabetic Retinopathy

    Article • Diabetes, August 2006, American Diabetes Association

    Dr Willard M Freeman

  87. Depletion of abundant proteins from non-human primate serum for biomarker studies

    Article • PROTEOMICS, May 2006, Wiley

    Dr Willard M Freeman

  88. APO-AII IS AN ELEVATED BIOMARKER OF CHRONIC NON-HUMAN PRIMATE ETHANOL SELF-ADMINISTRATION

    Article • Alcohol and Alcoholism, March 2006, Oxford University Press (OUP)

    Dr Willard M Freeman

  89. Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring rats

    Article • Journal of Neuroscience Research, May 2005, Wiley

    Dr Willard M Freeman

  90. Distinct proteomic profiles of amphetamine self-administration transitional states

    Article • The Pharmacogenomics Journal, January 2005, Nature

    Dr Willard M Freeman

  91. cDNA Arrays in Alcohol-Related Pathology: Methods and Approaches

    Article • January 2005, Elsevier

    Dr Willard M Freeman

  92. Manganese-Induced Cytotoxicity in Dopamine-Producing Cells

    Article • NeuroToxicology, June 2004, Elsevier

    Dr Willard M Freeman

  93. Proteomics for Protein Expression Profiling in Neuroscience

    Article • Neurochemical Research, June 2004, Springer Science + Business Media

    Dr Willard M Freeman

  94. Alterations in ionotropic glutamate receptor subunits during binge cocaine self-administration and withdrawal in rats

    Article • Journal of Neurochemistry, May 2004, Wiley

    Dr Willard M Freeman

  95. Use of Microarray Technologies in Toxicology Research

    Article • NeuroToxicology, June 2003, Elsevier

    Dr Willard M Freeman

  96. Induction of GADD45 and GADD153 in Neuroblastoma Cells by Dopamine-Induced Toxicity

    Article • NeuroToxicology, December 2002, Elsevier

    Dr Willard M Freeman

  97. Changes in rat frontal cortex gene expression following chronic cocaine

    Article • Molecular Brain Research, July 2002, Elsevier

    Dr Willard M Freeman

  98. Article • Neurochemical Research, January 2002, Springer Science + Business Media

    Dr Willard M Freeman

  99. Experimental Design for Hybridization Array Analysis of Gene Expression

    Article • December 2001, Taylor & Francis

    Dr Willard M Freeman

  100. Chronic cocaine-mediated changes in non-human primate nucleus accumbens gene expression

    Article • Journal of Neurochemistry, December 2001, Wiley

    Dr Willard M Freeman

  101. Cocaine-responsive gene expression changes in rat hippocampus

    Article • Neuroscience, December 2001, Elsevier

    Dr Willard M Freeman

  102. Phosphorimager

    Article • April 2001, Wiley

    Dr Willard M Freeman

  103. A cocaine analog, 2β-propanoyl-3β-(4-tolyl)-tropane (PTT), reduces tyrosine hydroxylase in the mesolimbic dopamine pathway

    Article • Drug and Alcohol Dependence, December 2000, Elsevier

    Dr Willard M Freeman

  104. Panning for molecular gold

    Article • Trends in Biotechnology, June 1999, Elsevier

    Dr Willard M Freeman

  105. The maturation of nucleic acid technologies

    Article • Trends in Biotechnology, February 1999, Elsevier

    Dr Willard M Freeman

  106. PCR-based apolipoprotein E genotype analysis from archival fixed brain

    Article • Journal of Neuroscience Methods, April 1998, Elsevier

    Dr Willard M Freeman

  107. Tyrosine mRNA is expressed in human substantia nigra

    Article • Molecular Brain Research, April 1997, Elsevier

    Dr Willard M Freeman

  108. Functional Genomic Analysis in Pain Research Using Hybridization Arrays

    Article • Springer Science + Business Media

    Dr Willard M Freeman

  109. Systematic Screening of Gene Expression Using a cDNA Macroarray

    Article • Springer Science + Business Media

    Dr Willard M Freeman