All Stories

  1. Design, Synthesis, and Evaluation of Novel Inhibitors of Aminoglycoside‐Resistance 16S Ribosomal RNA Methyltransferases

    Article • ChemMedChem, December 2025, Wiley

    Dr Graeme L Conn

  2. KNexPHENIX: A PHENIX-Based Workflow for Improving Cryo-EM and Crystallographic Structural Models

    Article • October 2025, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  3. Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI

    Article • August 2025, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  4. Basis for selective drug evasion of an aminoglycoside-resistance ribosomal RNA modification

    Article • Nature Communications, August 2025, Springer Science + Business Media

    Dr Graeme L Conn

  5. Discovery of First-in-Class Inhibitors Targeting a Pathogen-Associated Aminoglycoside-Resistance 16S rRNA Methyltransferase

    Article • ACS Infectious Diseases, July 2025, American Chemical Society (ACS)

    Dr Graeme L Conn

  6. Distant ribose 2′-O-methylation of 23S rRNA helix 69 pre-orders the capreomycin drug binding pocket at the ribosome subunit interface

    Article • Nucleic Acids Research, July 2025, Oxford University Press (OUP)

    Dr Graeme L Conn

  7. Regulation, structure, and activity of the Pseudomonas aeruginosa MexXY efflux system

    Article • Antimicrobial Agents and Chemotherapy, May 2025, ASM Journals

    Dr Graeme L Conn

  8. Determination of Pseudomonas aeruginosa MexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes

    Article • Microbiology Spectrum, March 2025, ASM Journals

    Dr Graeme L Conn

  9. Distant Ribose 2’-O-Methylation of 23S rRNA Helix 69 Pre-Orders the Capreomycin Drug Binding Pocket at the Ribosome Subunit Interface

    Article • November 2024, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  10. Evading resistance at the double

    Article • Nature Chemical Biology, October 2024, Springer Science + Business Media

    Dr Graeme L Conn

  11. Determination ofPseudomonas aeruginosaMexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes

    Article • October 2024, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  12. Structural and functional diversity of Resistance-Nodulation-Division (RND) efflux pump transporters with implications for antimicrobial resistance

    Article • Microbiology and Molecular Biology Reviews, September 2024, ASM Journals

    Dr Graeme L Conn

  13. Ensuring a sustained workforce to combat antibiotic resistance in the 21st century: the critical need for training the next-gen of scientists at the pre-doctoral level

    Article • Antimicrobial Agents and Chemotherapy, April 2024, ASM Journals

    Dr Graeme L Conn

  14. Di-berberine conjugates as chemical probes of Pseudomonas aeruginosa MexXY-OprM efflux function and inhibition

    Article • npj Antimicrobials and Resistance, October 2023, Springer Science + Business Media

    Dr Graeme L Conn

  15. 30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC

    Article • Proceedings of the National Academy of Sciences, June 2023, Proceedings of the National Academy of Sciences

    Dr Graeme L Conn

  16. Di-berberine conjugates as chemical probes ofPseudomonas aeruginosaMexXY-OprM efflux function and inhibition

    Article • March 2023, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  17. 30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC

    Article • March 2023, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  18. tRNA m1G9 modification depends on substrate-specific RNA conformational changes induced by the methyltransferase Trm10

    Article • February 2023, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  19. Ribosome-targeting antibiotics and resistance via ribosomal RNA methylation

    Article • RSC Medicinal Chemistry, January 2023, Royal Society of Chemistry

    Dr Graeme L Conn

  20. 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA

    Article • Proceedings of the National Academy of Sciences, March 2022, Proceedings of the National Academy of Sciences

    Dr Graeme L Conn

  21. Role of helical structure and dynamics in oligoadenylate synthetase 1 (OAS1) mismatch tolerance and activation by short dsRNAs

    Article • Proceedings of the National Academy of Sciences, January 2022, Proceedings of the National Academy of Sciences

    Dr Graeme L Conn

  22. 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA

    Article • November 2021, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  23. Targeted Redesign of Suramin Analogs for Novel Antimicrobial Lead Development

    Article • Journal of Chemical Information and Modeling, September 2021, American Chemical Society (ACS)

    Dr Graeme L Conn

  24. Targeted redesign of suramin analogs for novel antimicrobial lead development

    Article • May 2021, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  25. Antibiotic Substrate Selectivity of Pseudomonas aeruginosa MexY and MexB Efflux Systems Is Determined by a Goldilocks Affinity

    Article • Antimicrobial Agents and Chemotherapy, July 2020, ASM Journals

    Dr Graeme L Conn

  26. Human OAS1 activation is highly dependent on both RNA sequence and context of activating RNA motifs

    Article • Nucleic Acids Research, June 2020, Oxford University Press (OUP)

    Dr Graeme L Conn

  27. Substrate recognition by the Pseudomonas aeruginosa EF-Tu–modifying methyltransferase EftM

    Article • Journal of Biological Chemistry, November 2019, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  28. Functionally critical residues in the aminoglycoside resistance-associated methyltransferase RmtC play distinct roles in 30S substrate recognition

    Article • Journal of Biological Chemistry, October 2019, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  29. Substrate recognition by the Pseudomonas aeruginosa EF-Tu methyltransferase EftM

    Article • September 2019, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  30. Dysregulation of M segment gene expression contributes to influenza A virus host restriction

    Article • PLoS Pathogens, August 2019, PLOS

    Dr Graeme L Conn

  31. Critical residues in the aminoglycoside-resistance 16S rRNA (m7G1405) methyltransferase RmtC play distinct roles in 30S substrate recognition

    Article • July 2019, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  32. Trimethylation of Elongation Factor-Tu by the Dual Thermoregulated Methyltransferase EftM Does Not Impact Its Canonical Function in Translation

    Article • Scientific Reports, March 2019, Springer Science + Business Media

    Dr Graeme L Conn

  33. A human cellular noncoding RNA activates the antiviral protein 2’–5’-oligoadenylate synthetase 1

    Article • Journal of Biological Chemistry, August 2018, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  34. Substrate Recognition and Modification by a Pathogen-Associated Aminoglycoside Resistance 16S rRNA Methyltransferase

    Article • Antimicrobial Agents and Chemotherapy, March 2017, ASM Journals

    Dr Graeme L Conn

  35. Human non-coding RNA 886 (nc886) adopts two structurally distinct conformers that are functionally opposing regulators of PKR

    Article • RNA, January 2017, Cold Spring Harbor Laboratory Press

    Dr Graeme L Conn

  36. A Novel Motif for S-adenosyl-L-methionine Binding by the Ribosomal RNA Methyltransferase TlyA from Mycobacterium tuberculosis

    Article • Journal of Biological Chemistry, December 2016, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  37. Adenovirus VA RNA: An essential pro-viral non-coding RNA

    Article • Virus Research, January 2016, Elsevier

    Dr Graeme L Conn

  38. Functional dichotomy in the 16S rRNA (m 1 A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization

    Article • Nucleic Acids Research, November 2015, Oxford University Press (OUP)

    Dr Graeme L Conn

  39. Heterologous Expression and Functional Characterization of the Exogenously Acquired Aminoglycoside Resistance Methyltransferases RmtD, RmtD2, and RmtG

    Article • Antimicrobial Agents and Chemotherapy, November 2015, ASM Journals

    Dr Graeme L Conn

  40. The Regulatory and Kinase Domains but Not the Interdomain Linker Determine Human Double-stranded RNA-activated Kinase (PKR) Sensitivity to Inhibition by Viral Non-coding RNAs

    Article • Journal of Biological Chemistry, October 2015, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  41. The Pathogen-Derived Aminoglycoside Resistance 16S rRNA Methyltransferase NpmA Possesses Dual m1A1408/m1G1408 Specificity

    Article • Antimicrobial Agents and Chemotherapy, September 2015, ASM Journals

    Dr Graeme L Conn

  42. 30S Subunit-Dependent Activation of the Sorangium cellulosum So ce56 Aminoglycoside Resistance-Conferring 16S rRNA Methyltransferase Kmr

    Article • Antimicrobial Agents and Chemotherapy, March 2015, ASM Journals

    Dr Graeme L Conn

  43. A novel RNA molecular signature for activation of 2′-5′ oligoadenylate synthetase-1

    Article • Nucleic Acids Research, December 2014, Oxford University Press (OUP)

    Dr Graeme L Conn

  44. Plasmid Template Design and In Vitro Transcription of Short RNAs Within a “Structure Cassette” for Structure Probing Experiments

    Article • August 2012, Springer Science + Business Media

    Dr Graeme L Conn

  45. General Protocols for Preparation of Plasmid DNA Template, RNA In Vitro Transcription, and RNA Purification by Denaturing PAGE

    Article • August 2012, Springer Science + Business Media

    Dr Graeme L Conn

  46. Cis-Acting Ribozymes for the Production of RNA In Vitro Transcripts with Defined 5′ and 3′ Ends

    Article • August 2012, Springer Science + Business Media

    Dr Graeme L Conn

  47. Identification of new inhibitors of protein kinase R guided by statistical modeling

    Article • Bioorganic & Medicinal Chemistry Letters, July 2011, Elsevier

    Dr Graeme L Conn

  48. Reduced Sweetness of a Monellin (MNEI) Mutant Results from Increased Protein Flexibility and Disruption of a Distant Poly-(L-Proline) II Helix

    Article • Chemical Senses, February 2011, Oxford University Press (OUP)

    Dr Graeme L Conn

  49. Expression, purification and crystallization of adenosine 1408 aminoglycoside-resistance rRNA methyltransferases for structural studies

    Article • Protein Expression and Purification, January 2011, Elsevier

    Dr Graeme L Conn

  50. Magnesium-Dependent Interaction of PKR with Adenovirus VAI

    Article • Journal of Molecular Biology, October 2010, Elsevier

    Dr Graeme L Conn

  51. Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria

    Article • Nucleic Acids Research, July 2010, Oxford University Press (OUP)

    Dr Graeme L Conn

  52. Heterologous Escherichia coli Expression, Purification and Characterization of the GrmA Aminoglycoside-Resistance Methyltransferase

    Article • The Protein Journal, September 2009, Springer Science + Business Media

    Dr Graeme L Conn

  53. The PKR-binding domain of adenovirus VA RNAI exists as a mixture of two functionally non-equivalent structures

    Article • Nucleic Acids Research, July 2009, Oxford University Press (OUP)

    Dr Graeme L Conn

  54. Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics

    Article • Nucleic Acids Research, July 2009, Oxford University Press (OUP)

    Dr Graeme L Conn

  55. Structure of the Thiostrepton Resistance Methyltransferase{middle dot}S-Adenosyl-L-methionine Complex and Its Interaction with Ribosomal RNA

    Article • Journal of Biological Chemistry, April 2009, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  56. Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site

    Article • Research in Microbiology, November 2008, Elsevier

    Dr Graeme L Conn

  57. Critical Residues for Cofactor Binding and Catalytic Activity in the Aminoglycoside Resistance Methyltransferase Sgm

    Article • Journal of Bacteriology, June 2008, ASM Journals

    Dr Graeme L Conn

  58. Systematic Deletion of the Adenovirus-associated RNAI Terminal Stem Reveals a Surprisingly Active RNA Inhibitor of Double-stranded RNA-activated Protein Kinase

    Article • Journal of Biological Chemistry, April 2008, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Graeme L Conn

  59. Analysis of adenovirus VA RNAI structure and stability using compensatory base pair modifications

    Article • Nucleic Acids Research, January 2008, Oxford University Press (OUP)

    Dr Graeme L Conn

  60. Recombinant RNA expression

    Article • Nature Chemical Biology, July 2007, Springer Science + Business Media

    Dr Graeme L Conn

  61. Raman and Raman optical activity (ROA) analysis of RNA structural motifs in Domain I of the EMCV IRES

    Article • Nucleic Acids Research, January 2007, Oxford University Press (OUP)

    Dr Graeme L Conn

  62. Optimization of a Ribosomal Structural Domain by Natural Selection †

    Article • Biochemistry, May 2006, American Chemical Society (ACS)

    Dr Graeme L Conn

  63. Expression and Purification of Functional Ligand-Binding Domains of T1R3 Taste Receptors

    Article • Chemical Senses, April 2006, Oxford University Press (OUP)

    Dr Graeme L Conn

  64. Distinct Contributions of T1R2 and T1R3 Taste Receptor Subunits to the Detection of Sweet Stimuli

    Article • Current Biology, November 2005, Elsevier

    Dr Graeme L Conn

  65. Coevolution of Protein and RNA Structures within a Highly Conserved Ribosomal Domain

    Article • Chemistry & Biology, February 2005, Elsevier

    Dr Graeme L Conn

  66. NMR studies of the structure and Mg2+ binding properties of a conserved RNA motif of EMCV picornavirus IRES element

    Article • Nucleic Acids Research, September 2004, Oxford University Press (OUP)

    Dr Graeme L Conn

  67. Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G

    Article • Journal of Virology, December 2003, ASM Journals

    Professor Graham J. Belsham, Dr Graeme L Conn

  68. General plasmids for producing RNA in vitro transcripts with homogeneous ends

    Article • Nucleic Acids Research, August 2003, Oxford University Press (OUP)

    Dr Graeme L Conn

  69. The solution structure of a DNAmiddle dotRNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications

    Article • Nucleic Acids Research, May 2003, Oxford University Press (OUP)

    Dr Graeme L Conn

  70. A Compact RNA Tertiary Structure Contains a Buried Backbone–K+ Complex

    Article • Journal of Molecular Biology, May 2002, Elsevier

    Dr Graeme L Conn, Dr Vinod K Misra, Dr Eaton E Lattman