All Stories

  1. The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex

    Article • Molecular Microbiology, August 2019, Wiley

    Prof Frank Sargent

  2. The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complex

    Article • July 2019, Cold Spring Harbor Laboratory Press

    Prof Frank Sargent

  3. Controlling and co-ordinating chitinase secretion in a Serratia marcescens population

    Article • May 2019, Cold Spring Harbor Laboratory Press

    Prof Frank Sargent

  4. The structure of hydrogenase-2 from Escherichia coli : implications for H 2 -driven proton pumping

    Article • Biochemical Journal, March 2018, Portland Press Ltd.

    Prof Frank Sargent

  5. Structure and activity of ChiX: a peptidoglycan hydrolase required for chitinase secretion by Serratia marcescens

    Article • Biochemical Journal, December 2017, Portland Press Ltd.

    Prof Frank Sargent

  6. Identification of a stable complex between a [NiFe]-hydrogenase catalytic subunit and its maturation protease

    Article • FEBS Letters, January 2017, Wiley

    Prof Frank Sargent

  7. Biosynthesis of selenate reductase in Salmonella enterica: critical roles for the signal peptide and DmsD

    Article • Microbiology, December 2016, Society for General Microbiology

    Prof Frank Sargent

  8. Design and characterisation of synthetic operons for biohydrogen technology

    Article • Archives of Microbiology, November 2016, Springer Science + Business Media

    Prof Frank Sargent

  9. Biosynthesis of Salmonella enterica [NiFe]-hydrogenase-5: probing the roles of system-specific accessory proteins

    Article • JBIC Journal of Biological Inorganic Chemistry, August 2016, Springer Science + Business Media

    Prof Frank Sargent

  10. Hydrogen activation by [NiFe]-hydrogenases

    Article • Biochemical Society Transactions, June 2016, Portland Press Ltd.

    Prof Frank Sargent

  11. Exploring the directionality ofEscherichia coliformate hydrogenlyase: a membrane-bound enzyme capable of fixing carbon dioxide to organic acid

    Article • MicrobiologyOpen, May 2016, Wiley

    Prof Frank Sargent

  12. A Novel Aerobic Mechanism for Reductive Palladium Biomineralization and Recovery byEscherichia coli

    Article • Geomicrobiology Journal, February 2016, Taylor & Francis

    Prof Jonathan Lloyd, Prof Frank Sargent

  13. How the oxygen tolerance of a [NiFe]-hydrogenase depends on quaternary structure

    Article • JBIC Journal of Biological Inorganic Chemistry, February 2016, Springer Science + Business Media

    Prof Frank Sargent

  14. The Model [NiFe]-Hydrogenases of Escherichia coli

    Article • January 2016, Elsevier

    Prof Frank Sargent

  15. Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli

    Article • Biotechnology Reports, December 2015, Elsevier

    Prof Frank Sargent

  16. Mechanism of hydrogen activation by [NiFe] hydrogenases

    Article • Nature Methods, November 2015, Nature

    Prof Frank Sargent

  17. Dissection and engineering of theEscherichia coliformate hydrogenlyase complex

    Article • FEBS Letters, September 2015, Wiley

    Prof Frank Sargent

  18. SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells

    Article • Metallomics, January 2015, Oxford University Press (OUP)

    Dr Constanze Pinske, Prof Frank Sargent

  19. A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens

    Article • The Journal of Cell Biology, December 2014, Rockefeller University Press

    Prof Frank Sargent

  20. Physiology and Bioenergetics of [NiFe]-Hydrogenase 2-Catalyzed H2-Consuming and H2-Producing Reactions in Escherichia coli

    Article • Journal of Bacteriology, November 2014, ASM Journals

    Professor Gary Sawers, Dr Constanze Pinske, Prof Frank Sargent

  21. Bacterial formate hydrogenlyase complex

    Article • Proceedings of the National Academy of Sciences, August 2014, Proceedings of the National Academy of Sciences

    Prof Frank Sargent

  22. How oxygen reacts with oxygen-tolerant respiratory [NiFe]-hydrogenases

    Article • Proceedings of the National Academy of Sciences, April 2014, Proceedings of the National Academy of Sciences

    Prof Frank Sargent

  23. How the structure of the large subunit controls function in an oxygen-tolerant [NiFe]-hydrogenase

    Article • Biochemical Journal, March 2014, Portland Press Ltd.

    Prof Frank Sargent

  24. Transforming an oxygen-tolerant [NiFe] uptake hydrogenase into a proficient, reversible hydrogen producer

    Article • Energy & Environmental Science, January 2014, Royal Society of Chemistry

    Prof Frank Sargent

  25. Characterization of a periplasmic nitrate reductase in complex with its biosynthetic chaperone

    Article • FEBS Journal, December 2013, Wiley

    Prof Frank Sargent

  26. A regulatory domain controls the transport activity of a twin-arginine signal peptide

    Article • FEBS Letters, September 2013, Wiley

    Prof Frank Sargent

  27. Signal peptide etiquette during assembly of a complex respiratory enzyme

    Article • Molecular Microbiology, September 2013, Wiley

    Prof Frank Sargent

  28. A synthetic system for expression of components of a bacterial microcompartment

    Article • Microbiology, September 2013, Society for General Microbiology

    Prof Frank Sargent

  29. Characterization of a pre-export enzyme–chaperone complex on the twin-arginine transport pathway

    Article • Biochemical Journal, May 2013, Portland Press Ltd.

    Prof Frank Sargent

  30. Principles of Sustained Enzymatic Hydrogen Oxidation in the Presence of Oxygen – The Crucial Influence of High Potential Fe–S Clusters in the Electron Relay of [NiFe]-Hydrogenases

    Article • Journal of the American Chemical Society, February 2013, American Chemical Society (ACS)

    Prof Frank Sargent

  31. Crystal Structure of the O 2 -Tolerant Membrane-Bound Hydrogenase 1 from Escherichia coli in Complex with Its Cognate Cytochrome b

    Article • Structure, January 2013, Elsevier

    Prof Frank Sargent

  32. Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of Escherichia coli K-12

    Article • BMC Microbiology, July 2012, Springer Science + Business Media

    Professor Gary Sawers, Dr Constanze Pinske, Prof Frank Sargent

  33. The hows and whys of aerobic H2 metabolism

    Article • Current Opinion in Chemical Biology, April 2012, Elsevier

    Prof Frank Sargent

  34. Overlapping transport and chaperone‐binding functions within a bacterial twin‐arginine signal peptide

    Article • Molecular Microbiology, February 2012, Wiley

    Professor Geerten W. Vuister, Prof Frank Sargent

  35. Conserved Signal Peptide Recognition Systems across the Prokaryotic Domains

    Article • Biochemistry, February 2012, American Chemical Society (ACS)

    Prof Frank Sargent

  36. Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12

    Article • Microbiology, January 2012, Society for General Microbiology

    Professor Gary Sawers, Dr Constanze Pinske, Prof Frank Sargent

  37. Oxygen-Tolerant [NiFe]-Hydrogenases: The Individual and Collective Importance of Supernumerary Cysteines at the Proximal Fe-S Cluster

    Article • Journal of the American Chemical Society, October 2011, American Chemical Society (ACS)

    Prof Frank Sargent

  38. HowSalmonellaoxidises H2under aerobic conditions

    Article • FEBS Letters, August 2011, Wiley

    Prof Frank Sargent

  39. Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron–sulfur cluster-containing small subunit

    Article • Archives of Microbiology, June 2011, Springer Science + Business Media

    Professor Gary Sawers, Dr Constanze Pinske, Prof Frank Sargent

  40. Characterisation of the membrane-extrinsic domain of the TatB component of the twin arginine protein translocase

    Article • FEBS Letters, January 2011, Wiley

    Prof Frank Sargent

  41. The Tat Protein Export Pathway

    Article • EcoSal Plus, September 2010, ASM Journals

    Prof Frank Sargent

  42. Towards an integrated system for bio-energy: hydrogen production by Escherichia coli and use of palladium-coated waste cells for electricity generation in a fuel cell

    Article • Biotechnology Letters, September 2010, Springer Science + Business Media

    Prof Frank Sargent

  43. How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.

    Article • Journal of Biological Chemistry, June 2010, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Frank Sargent

  44. Involvement of hydrogenases in the formation of highly catalytic Pd(0) nanoparticles by bioreduction of Pd(II) using Escherichia coli mutant strains

    Article • Microbiology, June 2010, Society for General Microbiology

    Prof Frank Sargent

  45. Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping

    Article • FEBS Journal, January 2010, Wiley

    Prof Frank Sargent

  46. Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli

    Article • January 2010, Springer Science + Business Media

    Prof Frank Sargent

  47. HowEscherichia coliIs Equipped to Oxidize Hydrogen under Different Redox Conditions

    Article • Journal of Biological Chemistry, November 2009, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Frank Sargent

  48. Water−Gas Shift Reaction Catalyzed by Redox Enzymes on Conducting Graphite Platelets

    Article • Journal of the American Chemical Society, October 2009, American Chemical Society (ACS)

    Prof Frank Sargent

  49. Proteolytic processing of Escherichia coli twin-arginine signal peptides by LepB

    Article • Archives of Microbiology, October 2009, Springer Science + Business Media

    Prof Frank Sargent

  50. Remnant signal peptides on non-exported enzymes: implications for the evolution of prokaryotic respiratory chains

    Article • Microbiology, September 2009, Society for General Microbiology

    Prof Frank Sargent

  51. Plenary Lectures

    Article • FEBS Journal, June 2009, Wiley

    Prof Frank Sargent

  52. A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12

    Article • Archives of Microbiology, May 2009, Springer Science + Business Media

    Prof Frank Sargent

  53. Biorecovery of Precious Metals from Wastes and Conversion into Fuel Cell Catalyst for Electricity Production

    Article • Advanced Materials Research, May 2009, Trans Tech Publications

    Prof Frank Sargent

  54. The Escherichia coli Cell Division Protein and Model Tat Substrate SufI (FtsP) Localizes to the Septal Ring and Has a Multicopper Oxidase-Like Structure

    Article • Journal of Molecular Biology, February 2009, Elsevier

    Susan M Lea, Prof Frank Sargent

  55. Features of a twin-arginine signal peptide required for recognition by a Tat proofreading chaperone

    Article • FEBS Letters, November 2008, Wiley

    Prof Frank Sargent

  56. Biosynthesis of the respiratory formate dehydrogenases from Escherichia coli: characterization of the FdhE protein

    Article • Archives of Microbiology, August 2008, Springer Science + Business Media

    Prof Frank Sargent

  57. Escherichia coli tat mutant strains are able to transport maltose in the absence of an active malE gene

    Article • Archives of Microbiology, April 2008, Springer Science + Business Media

    Prof Frank Sargent

  58. Dissecting the roles ofEscherichia colihydrogenases in biohydrogen production

    Article • FEMS Microbiology Letters, January 2008, Oxford University Press (OUP)

    Prof Frank Sargent

  59. The twin-arginine transport system: moving folded proteins across membranes

    Article • Biochemical Society Transactions, November 2007, Portland Press Ltd.

    Prof Frank Sargent

  60. Look on the positive side! The orientation, identification and bioenergetics of ‘Archaeal’ membrane-bound nitrate reductases

    Article • FEMS Microbiology Letters, November 2007, Oxford University Press (OUP)

    Professor MARIA-JOSE BONETE, Dr Rosa María Martínez-Espinosa, Prof Frank Sargent

  61. Structural diversity in twin-arginine signal peptide-binding proteins

    Article • Proceedings of the National Academy of Sciences, October 2007, Proceedings of the National Academy of Sciences

    Professor Geerten W. Vuister, Prof Frank Sargent

  62. TatBC, TatB, and TatC form structurally autonomous units within the twin arginine protein transport system ofEscherichia coli

    Article • FEBS Letters, July 2007, Wiley

    Susan M Lea, Prof Frank Sargent

  63. Constructing the wonders of the bacterial world: biosynthesis of complex enzymes

    Article • Microbiology, March 2007, Society for General Microbiology

    Prof Frank Sargent

  64. Subunit composition and in vivo substrate-binding characteristics of Escherichia coli Tat protein complexes expressed at native levels

    Article • FEBS Journal, November 2006, Wiley

    Prof Frank Sargent

  65. Inactivation of theEscherichia coliK-12 twin-arginine translocation system promotes increased hydrogen production

    Article • FEMS Microbiology Letters, September 2006, Oxford University Press (OUP)

    Prof Frank Sargent

  66. Pathfinders and trailblazers: a prokaryotic targeting system for transport of folded proteins

    Article • FEMS Microbiology Letters, January 2006, Oxford University Press (OUP)

    Prof Frank Sargent

  67. Signal peptide–chaperone interactions on the twin-arginine protein transport pathway

    Article • Proceedings of the National Academy of Sciences, June 2005, Proceedings of the National Academy of Sciences

    Prof Frank Sargent, Professor Raymond J. Turner

  68. Protein targeting by the bacterial twin-arginine translocation (Tat) pathway

    Article • Current Opinion in Microbiology, April 2005, Elsevier

    Prof Frank Sargent

  69. Export of complex cofactor-containing proteins by the bacterial Tat pathway

    Article • Trends in Microbiology, April 2005, Elsevier

    Prof Frank Sargent

  70. Common principles in the biosynthesis of diverse enzymes

    Article • Biochemical Society Transactions, February 2005, Portland Press Ltd.

    Prof Frank Sargent

  71. Chaperones involved in assembly and export ofN-oxide reductases

    Article • Biochemical Society Transactions, February 2005, Portland Press Ltd.

    Prof Frank Sargent

  72. Coordinating assembly and export of complex bacterial proteins

    Article • The EMBO Journal, September 2004, Wiley

    Prof Frank Sargent

  73. Sequence analysis of bacterial redox enzyme maturation proteins (REMPs)

    Article • Canadian Journal of Microbiology, April 2004, Canadian Science Publishing

    Prof Frank Sargent, Professor Raymond J. Turner

  74. Light traffic: photo-crosslinking a novel transport system

    Article • Trends in Biochemical Sciences, February 2004, Elsevier

    Prof Frank Sargent

  75. A subset of bacterial inner membrane proteins integrated by the twin-arginine translocase

    Article • Molecular Microbiology, August 2003, Wiley

    Prof Frank Sargent

  76. Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins

    Article • FEBS Letters, July 2003, Wiley

    Prof Frank Sargent

  77. The Tat protein translocation pathway and its role in microbial physiology

    Article • January 2003, Elsevier

    Prof Frank Sargent

  78. Regulation of the Hydrogenase-4 Operon of Escherichia coli by the σ54-Dependent Transcriptional Activators FhlA and HyfR

    Article • Journal of Bacteriology, December 2002, ASM Journals

    Prof Frank Sargent

  79. How bacteria get energy from hydrogen: a genetic analysis of periplasmic hydrogen oxidation in Escherichia coli

    Article • International Journal of Hydrogen Energy, November 2002, Elsevier

    Prof Frank Sargent

  80. Oligomeric Properties and Signal Peptide Binding by Escherichia coli Tat Protein Transport Complexes

    Article • Journal of Molecular Biology, October 2002, Elsevier

    Prof Frank Sargent

  81. Assembly of membrane-bound respiratory complexes by the Tat protein-transport system

    Article • Archives of Microbiology, August 2002, Springer Science + Business Media

    Prof Frank Sargent

  82. Functional complexity of the twin-arginine translocase TatC component revealed by site-directed mutagenesis

    Article • Molecular Microbiology, March 2002, Wiley

    Prof Frank Sargent

  83. Behaviour of topological marker proteins targeted to the Tat protein transport pathway

    Article • Molecular Microbiology, February 2002, Wiley

    Prof Frank Sargent

  84. A genetic screen for suppressors of Escherichia coli Tat signal peptide mutations establishes a critical role for the second arginine within the twin-arginine motif

    Article • Archives of Microbiology, December 2001, Springer Science + Business Media

    Prof Frank Sargent

  85. Membrane interactions and self-association of the TatA and TatB components of the twin-arginine translocation pathway

    Article • FEBS Letters, September 2001, Wiley

    Prof Frank Sargent

  86. Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure

    Article • European Journal of Biochemistry, June 2001, Wiley

    Prof Frank Sargent

  87. A marriage of bacteriology with cell biology results in twin arginines

    Article • Trends in Microbiology, May 2001, Elsevier

    Prof Frank Sargent

  88. Constitutive Expression of Escherichia coli tat Genes Indicates an Important Role for the Twin-Arginine Translocase during Aerobic and Anaerobic Growth

    Article • Journal of Bacteriology, March 2001, ASM Journals

    Professor Gary Sawers, Prof Frank Sargent

  89. Crystal Structure of the Molybdenum Cofactor Biosynthesis Protein MobA from Escherichia coli at Near-Atomic Resolution

    Article • Structure, November 2000, Elsevier

    Prof Frank Sargent

  90. A novel protein transport system involved in the biogenesis of bacterial electron transfer chains

    Article • Biochimica et Biophysica Acta (BBA) - Bioenergetics, August 2000, Elsevier

    Prof Frank Sargent

  91. TatD Is a Cytoplasmic Protein with DNase Activity

    Article • Journal of Biological Chemistry, March 2000, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Frank Sargent

  92. The Tat protein export pathway

    Article • Molecular Microbiology, January 2000, Wiley

    Prof Frank Sargent

  93. Sec-independent Protein Translocation inEscherichia coli

    Article • Journal of Biological Chemistry, December 1999, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Frank Sargent

  94. An Essential Component of a Novel Bacterial Protein Export System with Homologues in Plastids and Mitochondria

    Article • Journal of Biological Chemistry, July 1998, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Frank Sargent

  95. Overlapping functions of components of a bacterial Sec-independent protein export pathway

    Article • The EMBO Journal, July 1998, Wiley

    Prof Frank Sargent