All Stories

  1. Drastic alterations in the loop structure around colchicine upon complex formation with an engineered lipocalin indicate a conformational selection mechanism

    Article • Acta Crystallographica Section F Structural Biology Communications, August 2023, International Union of Crystallography

    Professor Arne Skerra

  2. Proline/alanine-rich sequence (PAS) polypeptides as an alternative to PEG precipitants for protein crystallization

    Article • Acta Crystallographica Section F Structural Biology Communications, July 2020, International Union of Crystallography

    Professor Arne Skerra

  3. An engineered lipocalin that tightly complexes the plant poison colchicine for use as antidote and in bioanalytical applications

    Article • Biological Chemistry, December 2018, De Gruyter

    Professor Arne Skerra

  4. Selection of an Anticalin® against the membrane form of Hsp70 via bacterial surface display and its theranostic application in tumour models

    Article • Biological Chemistry, November 2017, De Gruyter

    Professor Arne Skerra

  5. The polypeptide biophysics of proline/alanine-rich sequences (PAS): Recombinant biopolymers with PEG-like properties

    Article • Biopolymers, October 2017, Wiley

    Professor Arne Skerra

  6. Anticalins directed against vascular endothelial growth factor receptor 3 (VEGFR-3) with picomolar affinities show potential for medical therapy and in vivo imaging

    Article • Biological Chemistry, January 2017, De Gruyter

    Professor Arne Skerra

  7. The Menagerie of Human Lipocalins: A Natural Protein Scaffold for Molecular Recognition of Physiological Compounds

    Article • Accounts of Chemical Research, April 2015, American Chemical Society (ACS)

    Professor Arne Skerra

  8. The dimeric crystal structure of the human fertility lipocalin glycodelin reveals a protein scaffold for the presentation of complex glycans

    Article • Biochemical Journal, February 2015, Portland Press Ltd.

    Professor Arne Skerra

  9. High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation

    Article • mAbs, January 2015, Taylor & Francis

    Professor Arne Skerra

  10. The Strep-tag System for One-Step Affinity Purification of Proteins from Mammalian Cell Culture

    Article • January 2015, Springer Science + Business Media

    Professor Arne Skerra

  11. Design and Characterization of a Modular Membrane Protein Anchor to Functionalize the Moss Physcomitrella patens with Extracellular Catalytic and/or Binding Activities

    Article • ACS Synthetic Biology, December 2014, American Chemical Society (ACS)

    Professor Arne Skerra

  12. Structure of dimeric human glycodelin

    Article • December 2014, Protein Data Bank, Rutgers University

    Professor Arne Skerra

  13. ANTICALINS ® & PASYLATION ® : NEW CONCEPTS FOR BIOPHARMACEUTICAL DRUG DEVELOPMENT FROM PROTEIN DESIGN

    Article • November 2014, World Scientific Pub Co Pte Lt

    Professor Arne Skerra

  14. 73

    Article • Cytokine, November 2014, Elsevier

    Professor Arne Skerra

  15. Enhanced in vivo efficacy of a long-life type I Interferon superagonist in a mouse model of multiple sclerosis

    Article • Journal of Neuroimmunology, October 2014, Elsevier

    Professor Arne Skerra

  16. PAS-cal: A repetitive peptide sequence calibration standard for MALDI mass spectrometry

    Article • PROTEOMICS, September 2014, Wiley

    Professor Arne Skerra

  17. Enhanced in Vivo Efficacy of a Type I Interferon Superagonist with Extended Plasma Half-life in a Mouse Model of Multiple Sclerosis

    Article • Journal of Biological Chemistry, September 2014, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Arne Skerra

  18. Non-Antibody Scaffolds as Alternative Therapeutic Agents

    Article • August 2014, Wiley

    Professor Arne Skerra

  19. A Fab fragment directed against the neural cell adhesion molecule L1 enhances functional recovery after injury of the adult mouse spinal cord

    Article • Biochemical Journal, June 2014, Portland Press Ltd.

    Professor Arne Skerra

  20. PAS-cal: a Generic Recombinant Peptide Calibration Standard for Mass Spectrometry

    Article • Journal of the American Society for Mass Spectrometry, May 2014, American Chemical Society (ACS)

    Professor Arne Skerra

  21. Derivatization of Antibody Fab Fragments: A Designer Enzyme for Native Protein Modification

    Article • ChemBioChem, April 2014, Wiley

    Professor Arne Skerra

  22. Biochemical Analysis of Recombinant AlkJ from Pseudomonas putida Reveals a Membrane-Associated, Flavin Adenine Dinucleotide-Dependent Dehydrogenase Suitable for the Biosynthetic Production of Aliphatic Aldehydes

    Article • Applied and Environmental Microbiology, February 2014, ASM Journals

    Professor Arne Skerra

  23. A Photoswitchable Neurotransmitter Analogue Bound to Its Receptor

    Article • Biochemistry, December 2013, American Chemical Society (ACS)

    Professor Arne Skerra

  24. Sortase-catalyzed in vitro functionalization of a HER2-specific recombinant Fab for tumor targeting of the plant cytotoxin gelonin

    Article • mAbs, December 2013, Taylor & Francis

    Professor Arne Skerra

  25. Crystal structure of bovine butyrophilin

    Article • November 2013, Protein Data Bank, Rutgers University

    Professor Arne Skerra

  26. 113

    Article • Cytokine, September 2013, Elsevier

    Professor Arne Skerra

  27. Coupled Enzymatic Alcohol-to-Amine Conversion of Isosorbide using Engineered Transaminases and Dehydrogenases

    Article • ChemCatChem, August 2013, Wiley

    Professor Arne Skerra

  28. Crystallographic analysis and structure-guided engineering of NADPH-dependentRalstoniasp. Alcohol dehydrogenase toward NADH cosubstrate specificity

    Article • Biotechnology and Bioengineering, July 2013, Wiley

    Professor Arne Skerra

  29. Combinatorial Design of an Anticalin Directed against the Extra-Domain B for the Specific Targeting of Oncofetal Fibronectin

    Article • Journal of Molecular Biology, February 2013, Elsevier

    Professor Arne Skerra

  30. Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III amino- transferases that have biotechnological potential

    Article • Proteins Structure Function and Bioinformatics, January 2013, Wiley

    Professor Arne Skerra

  31. Deactivation of the E. coli pH Stress Sensor CadC by Cadaverine

    Article • Journal of Molecular Biology, November 2012, Elsevier

    Kirsten Dr. Jung, Professor Arne Skerra

  32. β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors

    Article • Biological Chemistry, November 2012, De Gruyter

    Professor Arne Skerra

  33. Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats

    Article • Toxicology and Applied Pharmacology, September 2012, Elsevier

    Professor Arne Skerra

  34. The crystal structure of human α 1 -microglobulin reveals a potential haem-binding site

    Article • Biochemical Journal, July 2012, Portland Press Ltd.

    Professor Arne Skerra

  35. High molecular mass radioimmunoconjugates are promising for intraperitoneal α-emitter immunotherapy due to prolonged retention in the peritoneum

    Article • Nuclear Medicine and Biology, July 2012, Elsevier

    Professor Arne Skerra

  36. NMR Structure of Lipoprotein YxeF from Bacillus subtilis Reveals a Calycin Fold and Distant Homology with the Lipocalin Blc from Escherichia coli

    Article • PLoS ONE, June 2012, PLOS

    Professor Arne Skerra

  37. Novel Serial Positive Enrichment Technology Enables Clinical Multiparameter Cell Sorting

    Article • PLoS ONE, April 2012, PLOS

    Professor Arne Skerra

  38. Half-Life Extension of Therapeutic Proteins via Genetic Fusion to Recombinant PEG Mimetics

    Article • February 2012, Wiley

    Professor Arne Skerra

  39. Anticalins

    Article • January 2012, Elsevier

    Professor Arne Skerra

  40. Amination of Ketones by Employing Two New (S)-Selective ω-Transaminases and the His-Tagged ω-TA from Vibrio fluvialis

    Article • European Journal of Organic Chemistry, December 2011, Wiley

    Professor Arne Skerra

  41. A Disulfide-Free Single-Domain VL Intrabody with Blocking Activity towards Huntingtin Reveals a Novel Mode of Epitope Recognition

    Article • Journal of Molecular Biology, December 2011, Elsevier

    Professor Arne Skerra

  42. Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment

    Article • Radiotherapy and Oncology, June 2011, Elsevier

    Professor Arne Skerra

  43. Biosynthesis of a Fluorescent Protein with Extreme Pseudo-Stokes Shift by Introducing a Genetically Encoded Non-Natural Amino Acid outside the Fluorophore

    Article • Journal of the American Chemical Society, March 2011, American Chemical Society (ACS)

    Professor Arne Skerra

  44. Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR‐like protein family

    Article • Protein Science, March 2011, Wiley

    Kirsten Dr. Jung, Professor Arne Skerra

  45. Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc

    Article • Acta Crystallographica Section D Biological Crystallography, November 2010, International Union of Crystallography

    Professor Arne Skerra

  46. Periplasmic Chaperones Used to Enhance Functional Secretion of Proteins in E. coli

    Article • November 2010, Springer Science + Business Media

    Professor Arne Skerra

  47. Engineering of an Orthogonal Aminoacyl-tRNA Synthetase for Efficient Incorporation of the Non-natural Amino Acid O-Methyl-L-tyrosine using Fluorescence-based Bacterial Cell Sorting

    Article • Journal of Molecular Biology, November 2010, Elsevier

    Professor Arne Skerra

  48. Incorporation of extra-domain B into fibronectin mediates local dimerization

    Article • Acta Crystallographica Section A Foundations of Crystallography, August 2010, International Union of Crystallography

    Professor Arne Skerra

  49. Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel

    Article • Nature Immunology, August 2010, Springer Science + Business Media

    Professor Marc Schmidt, Professor Arne Skerra

  50. High-throughput Sorting of an Anticalin Library via EspP-mediated Functional Display on the Escherichia coli Cell Surface

    Article • Journal of Molecular Biology, July 2010, Elsevier

    Professor Arne Skerra

  51. 757 Endoplasmic Reticulum Stress Responses Contribute to the Cytotoxic Phenotype of CD8αβ Intraepithelial Lymphocytes Through the Regulation Granzyme B Activity in Crohn's Disease-Like TNFΔ are Mice

    Article • Gastroenterology, May 2010, Elsevier

    Professor Arne Skerra

  52. Ifn gamma inhibits Wnt signaling during colitis.

    Article • Immunity, March 2010, Elsevier

    Professor Arne Skerra, Porfirio Nava

  53. Purification and Characterization of His-Tagged Antibody Fragments

    Article • January 2010, Springer Science + Business Media

    Professor Arne Skerra

  54. Purification and Analysis of Strep-tagged Antibody-Fragments

    Article • January 2010, Springer Science + Business Media

    Professor Arne Skerra

  55. Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay

    Article • January 2010, Springer Science + Business Media

    Professor Arne Skerra

  56. A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity

    Article • Acta Crystallographica Section D Biological Crystallography, September 2009, International Union of Crystallography

    Professor Arne Skerra

  57. NMR Structure and Dynamics of the Engineered Fluorescein-Binding Lipocalin FluA Reveal Rigidification of β-Barrel and Variable Loops upon Enthalpy-Driven Ligand Binding

    Article • Biochemistry, August 2009, American Chemical Society (ACS)

    Professor Arne Skerra

  58. Engineered protein scaffolds as next-generation antibody therapeutics

    Article • Current Opinion in Chemical Biology, June 2009, Elsevier

    Professor Arne Skerra

  59. High-Affinity Recognition of Lanthanide(III) Chelate Complexes by a Reprogrammed Human Lipocalin 2

    Article • Journal of the American Chemical Society, March 2009, American Chemical Society (ACS)

    Professor Arne Skerra

  60. Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli

    Article • Protein Science, December 2008, Wiley

    Professor Arne Skerra

  61. The 1.8-Å Crystal Structure of α1-Acid Glycoprotein (Orosomucoid) Solved by UV RIP Reveals the Broad Drug-Binding Activity of This Human Plasma Lipocalin

    Article • Journal of Molecular Biology, December 2008, Elsevier

    Professor Arne Skerra

  62. Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp

    Article • PROTEOMICS, December 2008, Wiley

    Professor Arne Skerra

  63. Structure of Lumazine Protein, an Optical Transponder of Luminescent Bacteria

    Article • Journal of Molecular Biology, September 2008, Elsevier

    Professor Arne Skerra

  64. DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis

    Article • European Journal of Pharmacology, June 2008, Elsevier

    Professor Arne Skerra

  65. Structural analysis of theL-alanoyl-D-glutamate endopeptidase domain ofListeriabacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family

    Article • Acta Crystallographica Section D Biological Crystallography, May 2008, International Union of Crystallography

    Professor Arne Skerra

  66. The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops

    Article • Proteins Structure Function and Bioinformatics, May 2008, Wiley

    Professor Arne Skerra

  67. Alternative binding proteins get mature: Rivalling antibodies

    Article • FEBS Journal, April 2008, Wiley

    Professor Arne Skerra

  68. Alternative binding proteins: Anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities

    Article • FEBS Journal, April 2008, Wiley

    Professor Arne Skerra

  69. The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site

    Article • Molecular Immunology, April 2008, Elsevier

    Professor Arne Skerra

  70. Tailoring Protein Scaffolds for Ligand Recognition

    Article • March 2008, Wiley

    Professor Arne Skerra

  71. Alternative non-antibody scaffolds for molecular recognition

    Article • Current Opinion in Biotechnology, August 2007, Elsevier

    Professor Arne Skerra

  72. The Crystal Structure of the Human (S100A8/S100A9)2 Heterotetramer, Calprotectin, Illustrates how Conformational Changes of Interacting α-Helices Can Determine Specific Association of Two EF-hand Proteins

    Article • Journal of Molecular Biology, July 2007, Elsevier

    Professor Arne Skerra

  73. Anticalins®: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies

    Article • Expert Review of Clinical Immunology, July 2007, Taylor & Francis

    Professor Arne Skerra

  74. The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins

    Article • Nature Protocols, June 2007, Springer Science + Business Media

    Professor Arne Skerra

  75. Biochemical characterization of the recombinant human Nogo-A ectodomain

    Article • FEBS Journal, April 2007, Wiley

    Professor Arne Skerra

  76. Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique

    Article • Journal of Molecular Recognition, January 2007, Wiley

    Professor Arne Skerra

  77. The Crystal Structure of the Bacteriophage PSA Endolysin Reveals a Unique Fold Responsible for Specific Recognition of Listeria Cell Walls

    Article • Journal of Molecular Biology, December 2006, Elsevier

    Professor Arne Skerra

  78. Comparative ligand-binding analysis of ten human lipocalins

    Article • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, February 2006, Elsevier

    Professor Arne Skerra

  79. Rational engineering of a fluorescein-binding anticalin for improved ligand affinity

    Article • Biological Chemistry, November 2005, De Gruyter

    Professor Arne Skerra

  80. Anticalins as an alternative to antibody technology

    Article • Expert Opinion on Biological Therapy, November 2005, Informa Healthcare

    Professor Arne Skerra

  81. Biochemistry in 2004

    Article • ChemInform, May 2005, Wiley

    Professor Arne Skerra

  82. Anticalins in Drug Development

    Article • BioDrugs, January 2005, Springer Science + Business Media

    Professor Arne Skerra

  83. Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture

    Article • Nature Structural & Molecular Biology, September 2004, Springer Science + Business Media

    Professor Arne Skerra

  84. Binding proteins from alternative scaffolds

    Article • Journal of Immunological Methods, July 2004, Elsevier

    Professor Arne Skerra

  85. A Novel Method for the N-Terminal Modification of Native Proteins

    Article • Bioconjugate Chemistry, March 2004, American Chemical Society (ACS)

    Professor Arne Skerra

  86. Construction of an Artificial Receptor Protein (“Anticalin”) Based on the Human Apolipoprotein D

    Article • ChemBioChem, January 2004, Wiley

    Professor Arne Skerra

  87. Crystallographic analysis of an “anticalin” with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region

    Article • Proteins Structure Function and Bioinformatics, August 2003, Wiley

    Professor Arne Skerra

  88. Structural Mechanism of Specific Ligand Recognition by a Lipocalin Tailored for the Complexation of Digoxigenin

    Article • Journal of Molecular Biology, July 2003, Elsevier

    Professor Arne Skerra

  89. Generation of anticalins with specificity for a nonsymmetric phthalic acid ester

    Article • Analytical Biochemistry, September 2002, Elsevier

    Professor Arne Skerra

  90. Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant — a so-called ‘anticalin’ — using a molecular random approach

    Article • Biophysical Chemistry, May 2002, Elsevier

    Professor Arne Skerra

  91. proBA complementation of an auxotrophic E. coli strain improves plasmid stability and expression yield during fermenter production of a recombinant antibody fragment

    Article • Gene, August 2001, Elsevier

    Professor Arne Skerra

  92. ‘Anticalins’: a new class of engineered ligand-binding proteins with antibody-like properties

    Article • Reviews in Molecular Biotechnology, June 2001, Elsevier

    Professor Arne Skerra

  93. Purification and Characterisation of His-Tagged Antibody Fragments

    Article • January 2001, Springer Science + Business Media

    Professor Arne Skerra

  94. Purification and Analysis of Strep-Tagged Antibody-Fragments

    Article • January 2001, Springer Science + Business Media

    Professor Arne Skerra

  95. Bacterially produced apolipoprotein D binds progesterone and arachidonic acid, but not bilirubin or E‐3M2H

    Article • Journal of Molecular Recognition, January 2001, Wiley

    Professor Arne Skerra

  96. Bacterially produced apolipoprotein D binds progesterone and arachidonic acid, but not bilirubin orE-3M2H

    Article • Journal of Molecular Recognition, January 2001, Wiley

    Professor Arne Skerra

  97. Lipocalins as a scaffold

    Article • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, October 2000, Elsevier

    Professor Arne Skerra

  98. Engineered protein scaffolds for molecular recognition

    Article • Journal of Molecular Recognition, July 2000, Wiley

    Professor Arne Skerra

  99. A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin11Edited by R. Huber

    Article • Journal of Molecular Biology, April 2000, Elsevier

    Professor Arne Skerra

  100. Engineered protein scaffolds for molecular recognition

    Article • Journal of Molecular Recognition, January 2000, Wiley

    Professor Arne Skerra

  101. [18] Use of the Strep- tag and streptavidin for detection and purification of recombinant proteins

    Article • January 2000, Elsevier

    Professor Arne Skerra

  102. Applications of a peptide ligand for streptavidin: the Strep-tag

    Article • Biomolecular Engineering, December 1999, Elsevier

    Professor Arne Skerra

  103. Use of Thiophilic Adsorption Chromatography for the One-Step Purification of a Bacterially Produced Antibody Fab Fragment without the Need for an Affinity Tag

    Article • Protein Expression and Purification, December 1999, Elsevier

    Professor Arne Skerra

  104. Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination

    Article • Journal of Immunological Methods, October 1999, Elsevier

    Professor Arne Skerra

  105. Intracellular Expression of a Cloned Antibody Fragment Interferes with Hepatitis B Virus Surface Antigen Secretion

    Article • Biochemical and Biophysical Research Communications, February 1999, Elsevier

    Professor Arne Skerra

  106. Immobilization of histidine-tagged proteins on gold surfaces using chelator thioalkanes

    Article • Biosensors and Bioelectronics, February 1999, Elsevier

    Professor Arne Skerra

  107. Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein

    Article • Gene, October 1998, Elsevier

    Professor Arne Skerra

  108. Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates

    Article • Journal of Immunological Methods, September 1998, Elsevier

    Professor Arne Skerra

  109. Sequence analysis and bacterial production of the anti-c-myc antibody 9E10: the VH domain has an extended CDR-H3 and exhibits unusual solubility

    Article • FEBS Letters, September 1997, Wiley

    Professor Arne Skerra

  110. The rational construction of an antibody against cystatin: lessons from the crystal structure of an artificial Fab Fragment

    Article • Journal of Molecular Biology, May 1997, Elsevier

    Professor Arne Skerra

  111. The Escherichia Coli-Derived Fab Fragment of the IgM/κ Antibody IN-1 Recognizes and Neutralizes Myelin-Associated Inhibitors of Neurite Growth

    Article • European Journal of Biochemistry, October 1996, Wiley

    Professor Arne Skerra

  112. A Zn(II)-binding site engineered into retinol-binding protein exhibits metal-ion specificity and allows highly efficient affinity purification with a newly designed metal ligand

    Article • Chemistry & Biology, August 1996, Elsevier

    Professor Arne Skerra

  113. Molecular Interaction Between the Strep-tag Affinity Peptide and its Cognate Target, Streptavidin

    Article • Journal of Molecular Biology, February 1996, Elsevier

    Professor Arne Skerra

  114. Fermenter production of an artificial fab fragment, rationally designed for the antigen cystatin, and its optimized crystallization through constant domain shuffling

    Article • Proteins Structure Function and Bioinformatics, December 1995, Wiley

    Professor Arne Skerra

  115. Engineered Fv Fragments as a Tool for the One-Step Purification of Integral Multisubunit Membrane Protein Complexes

    Article • Nature Biotechnology, February 1995, Springer Science + Business Media

    Professor Arne Skerra

  116. Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

    Article • Gene, December 1994, Elsevier

    Professor Arne Skerra

  117. Grafting of a High-Affinity Zn(II)-Binding Site on the .beta.-Barrel of Retinol-Binding Protein Results in Enhanced Folding Stability and Enables Simplified Purification

    Article • Biochemistry, November 1994, American Chemical Society (ACS)

    Professor Arne Skerra

  118. One-step affinity purification of bacterially produced proteins by means of the “Strep tag” and immobilized recombinant core streptavidin

    Article • Journal of Chromatography A, August 1994, Elsevier

    Professor Arne Skerra

  119. The de Novo Design of an Antibody Combining Site

    Article • Journal of Molecular Biology, April 1994, Elsevier

    Professor Arne Skerra

  120. A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments

    Article • Gene, April 1994, Elsevier

    Professor Arne Skerra

  121. The bilin-binding protein of Pieris brassicae. cDNA sequence and regulation of expression reveal distinct features of this insect pigment protein

    Article • European Journal of Biochemistry, February 1994, Wiley

    Professor Arne Skerra

  122. Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine

    Article • Journal of Chromatography A, December 1993, Elsevier

    Professor Arne Skerra

  123. Functional Expression of the Uncomplexed Serum Retinol-binding Protein in Escherichia coli

    Article • Journal of Molecular Biology, April 1993, Elsevier

    Professor Arne Skerra

  124. Bacterial expression of immunoglobulin fragments

    Article • Current Opinion in Immunology, January 1993, Elsevier

    Professor Arne Skerra

  125. The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment

    Article • Protein Engineering Design and Selection, January 1993, Oxford University Press (OUP)

    Professor Arne Skerra

  126. Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity

    Article • Nucleic Acids Research, January 1992, Oxford University Press (OUP)

    Professor Arne Skerra

  127. Filter screening of antibody Fab fragments secreted from individual bacterial colonies: Specific detection of antigen binding with a two-membrane system

    Article • Analytical Biochemistry, July 1991, Elsevier

    Professor Arne Skerra

  128. Colony assays for antibody fragments expressed in bacteria

    Article • Journal of Immunological Methods, June 1991, Elsevier

    Professor Arne Skerra

  129. The Functional Expression of Antibody Fv Fragments in Ischhuchia coli: Improved Vectors and a Generally Applicable Purification Technique

    Article • Nature Biotechnology, March 1991, Springer Science + Business Media

    Professor Arne Skerra

  130. Secretion and in vivo folding of the F ab fragment of the antibody McPC603 in Escherichia coli : influence of disulphides and cis -prolines

    Article • Protein Engineering Design and Selection, January 1991, Oxford University Press (OUP)

    Professor Arne Skerra

  131. Structural features of the McPC603 Fabfragment not defined in the X-ray structure

    Article • FEBS Letters, October 1990, Wiley

    Professor Arne Skerra

  132. [34] Expression of functional antibody Fv and Fab fragments in Escherichia coli

    Article • January 1989, Elsevier

    Professor Arne Skerra

  133. Structure and Dynamics of One Dimensional Ionic Solutions in Narrow Transmembrane Channels — A Molecular Dynamics Simulation Study —

    Article • January 1987, Springer Science + Business Media

    Professor Arne Skerra

  134. Non-Antibody Scaffolds

    Article • Wiley

    Professor Arne Skerra