All Stories

  1. The Substrate Versatility of Δ1-Pyrroline-5-carboxylate Reductase (ProC) from Escherichia coli

    Article • Molecules, January 2026, MDPI AG

    Alessio Peracchi

  2. The Pyridoxal-5′-Phosphate-Dependent Enzymes of Mycobacterium tuberculosis

    Article • ACS Infectious Diseases, January 2026, American Chemical Society (ACS)

    Alessio Peracchi

  3. A phosphoserine phosphatase variant present in the brain of Alzheimer's disease patients favors nuclear mistargeting

    Article • European Journal of Biochemistry, July 2025, Wiley

    Alessio Peracchi

  4. Genomic context analysis enables the discovery of an unusual NAD ‐dependent racemase in phosphonate catabolism

    Article • European Journal of Biochemistry, May 2025, Wiley

    Alessio Peracchi

  5. One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse

    Article • Nature Communications, April 2024, Springer Science + Business Media

    Alessio Peracchi

  6. Effect of l-serine and magnesium ions on the functional properties of human phosphoserine phosphatase and its pathogenetic variants

    Article • Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, March 2024, Elsevier

    Alessio Peracchi

  7. Deciphering the role of recurrent FAD-dependent enzymes in bacterial phosphonate catabolism

    Article • iScience, November 2023, Elsevier

    Alessio Peracchi

  8. The Microbial Degradation of Natural and Anthropogenic Phosphonates

    Article • Molecules, September 2023, MDPI AG

    Alessio Peracchi

  9. Phosphoserine Aminotransferase Pathogenetic Variants in Serine Deficiency Disorders: A Functional Characterization

    Article • Biomolecules, August 2023, MDPI AG

    Alessio Peracchi

  10. The human phosphorylated pathway: a multienzyme metabolic assembly for l‐serine biosynthesis

    Article • European Journal of Biochemistry, April 2023, Wiley

    Alessio Peracchi

  11. L‐serine biosynthesis in the human central nervous system: Structure and function of phosphoserine aminotransferase

    Article • Protein Science, March 2023, Wiley

    Alessio Peracchi

  12. Human cytosolic transaminases: side activities and patterns of discrimination towards physiologically available alternative substrates

    Article • Cellular and Molecular Life Sciences, July 2022, Springer Science + Business Media

    Alessio Peracchi

  13. Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases

    Article • Molecules, February 2022, MDPI AG

    Alessio Peracchi

  14. A Novel Assay for Phosphoserine Phosphatase Exploiting Serine Acetyltransferase as the Coupling Enzyme

    Article • Life, May 2021, MDPI AG

    Alessio Peracchi

  15. Biochemical and Biophysical Characterization of Recombinant Human 3-Phosphoglycerate Dehydrogenase

    Article • International Journal of Molecular Sciences, April 2021, MDPI AG

    Alessio Peracchi

  16. Discovery of a New, Recurrent Enzyme in Bacterial Phosphonate Degradation: (R)-1-Hydroxy-2-aminoethylphosphonate Ammonia-lyase

    Article • Biochemistry, April 2021, American Chemical Society (ACS)

    Alessio Peracchi

  17. The metalloprotein YhcH is an anomerase providing N-acetylneuraminate aldolase with the open form of its substrate

    Article • Journal of Biological Chemistry, January 2021, Elsevier

    Alessio Peracchi

  18. Birth of a pathway for sulfur metabolism in early amniote evolution

    Article • Nature Ecology & Evolution, June 2020, Springer Science + Business Media

    Alessio Peracchi

  19. The putative Escherichia coli dehydrogenase YjhC metabolises two dehydrated forms of N-acetylneuraminate produced by some sialidases

    Article • Bioscience Reports, June 2020, Portland Press Ltd.

    Alessio Peracchi

  20. Structural characterization of human O-phosphoethanolamine phospho-lyase

    Article • Acta Crystallographica Section F Structural Biology Communications, April 2020, International Union of Crystallography

    Alessio Peracchi

  21. Off to a slow start: Analyzing lag phases and accelerating rates in steady-state enzyme kinetics

    Article • Analytical Biochemistry, March 2020, Elsevier

    Alessio Peracchi

  22. Insights into DNA catalysis from structural and functional studies of the 8-17 DNAzyme

    Article • Organic & Biomolecular Chemistry, January 2020, Royal Society of Chemistry

    Alessio Peracchi

  23. The Limits of Enzyme Specificity and the Evolution of Metabolism

    Article • Trends in Biochemical Sciences, December 2018, Elsevier

    Alessio Peracchi

  24. Nit1 is a metabolite repair enzyme that hydrolyzes deaminated glutathione

    Article • Proceedings of the National Academy of Sciences, April 2017, Proceedings of the National Academy of Sciences

    Alessio Peracchi

  25. Local conformational changes in the 8–17 deoxyribozyme core induced by activating and inactivating divalent metal ions

    Article • Organic & Biomolecular Chemistry, January 2017, Royal Society of Chemistry

    Alessio Peracchi

  26. Dissecting the hybridization of oligonucleotides to structured complementary sequences

    Article • Biochimica et Biophysica Acta (BBA) - General Subjects, June 2016, Elsevier

    Alessio Peracchi

  27. A subfamily of PLP-dependent enzymes specialized in handling terminal amines

    Article • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, September 2015, Elsevier

    Alessio Peracchi

  28. Kinetic characterization of the humanO-phosphoethanolamine phospho-lyase reveals unconventional features of this specialized pyridoxal phosphate-dependent lyase

    Article • European Journal of Biochemistry, November 2014, Wiley

    Alessio Peracchi

  29. Regulation of human serine racemase activity and dynamics by halides, ATP and malonate

    Article • Amino Acids, October 2014, Springer Science + Business Media

    Alessio Peracchi

  30. Completing the folate biosynthesis pathway inPlasmodium falciparum:p-aminobenzoate is produced by a highly divergent promiscuous aminodeoxychorismate lyase

    Article • Biochemical Journal, October 2013, Portland Press Ltd.

    Alessio Peracchi

  31. ATP binding to human serine racemase is cooperative and modulated by glycine

    Article • European Journal of Biochemistry, September 2013, Wiley

    Alessio Peracchi, Andrea Mozzarelli

  32. Strict reaction and substrate specificity of AGXT2L1, the humanO-phosphoethanolamine phospho-lyase

    Article • IUBMB Life, June 2013, Wiley

    Alessio Peracchi

  33. Serine racemase: a key player in neuron activity and in neuropathologies

    Article • Frontiers in Bioscience, January 2013, Frontiers in Bioscience

    Alessio Peracchi

  34. Exploring and exploiting allostery: Models, evolution, and drug targeting

    Article • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, August 2011, Elsevier

    Alessio Peracchi

  35. An aminotransferase branch point connects purine catabolism to amino acid recycling

    Article • Nature Chemical Biology, September 2010, Springer Science + Business Media

    Alessio Peracchi

  36. Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucG) from Bacillus subtilis

    Article • September 2010, Protein Data Bank, Rutgers University

    Alessio Peracchi

  37. Pyridoxal 5′-Phosphate-Dependent Enzymes: Catalysis, Conformation, and Genomics

    Article • January 2010, Elsevier

    Alessio Peracchi

  38. Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism

    Article • Biochemical Journal, September 2009, Portland Press Ltd.

    Alessio Peracchi

  39. The B6 database: a tool for the description and classification of vitamin B6-dependent enzymatic activities and of the corresponding protein families

    Article • BMC Bioinformatics, January 2009, Springer Science + Business Media

    Alessio Peracchi

  40. How (and Why) to Revive a Dead Enzyme: The Power of Chemical Rescue

    Article • January 2008, Bentham Science Publishers

    Alessio Peracchi

  41. How (and Why) to Revive a Dead Enzyme: The Power of Chemical Rescue

    Article • Current Chemical Biology, January 2008, Bentham Science Publishers

    Alessio Peracchi

  42. The Advantages of Being Locked

    Article • Journal of Biological Chemistry, December 2007, Elsevier

    Alessio Peracchi

  43. Efficient and Selective Cleavage of RNA Oligonucleotides by Calix[4]arene-Based Synthetic Metallonucleases

    Article • Journal of the American Chemical Society, September 2007, American Chemical Society (ACS)

    Professor Alessandro Casnati, Alessio Peracchi

  44. A threonine synthase homolog from a mammalian genome

    Article • Biochemical and Biophysical Research Communications, December 2006, Elsevier

    Alessio Peracchi

  45. A Mutational Analysis of the 8–17 Deoxyribozyme Core

    Article • Journal of Molecular Biology, September 2005, Elsevier

    Alessio Peracchi

  46. DNA Catalysis: Potential, Limitations, Open Questions

    Article • ChemBioChem, July 2005, Wiley

    Alessio Peracchi

  47. Kinetic and thermodynamic characterization of the RNA-cleaving 8-17 deoxyribozyme

    Article • Nucleic Acids Research, February 2004, Oxford University Press (OUP)

    Alessio Peracchi

  48. Domain Organization of Phytochelatin Synthase: FUNCTIONAL PROPERTIES OF TRUNCATED ENZYME SPECIES IDENTIFIED BY LIMITED PROTEOLYSIS

    Article • Journal of Biological Chemistry, January 2004, American Society for Biochemistry & Molecular Biology (ASBMB)

    Alessio Peracchi

  49. Prospects for antiviral ribozymes and deoxyribozymes

    Article • Reviews in Medical Virology, January 2004, Wiley

    Alessio Peracchi

  50. A genomic overview of pyridoxal-phosphate-dependent enzymes

    Article • EMBO Reports, September 2003, Wiley

    Alessio Peracchi

  51. Catalysis and electron transfer in protein crystals: the binary and ternary complexes of methylamine dehydrogenase with electron acceptors

    Article • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, April 2003, Elsevier

    Alessio Peracchi

  52. Enzyme catalysis: removing chemically ‘essential’ residues by site-directed mutagenesis

    Article • Trends in Biochemical Sciences, August 2001, Elsevier

    Alessio Peracchi

  53. Preferential Activation of the 8-17 Deoxyribozyme by Ca2+ Ions. EVIDENCE FOR THE IDENTITY OF 8-17 WITH THE CATALYTIC DOMAIN OF THE MG5 DEOXYRIBOZYME

    Article • Journal of Biological Chemistry, April 2000, American Society for Biochemistry & Molecular Biology (ASBMB)

    Alessio Peracchi

  54. Effect of pH and Monovalent Cations on the Formation of Quinonoid Intermediates of the Tryptophan Synthase  2 2 Complex in Solution and in the Crystal

    Article • Journal of Biological Chemistry, March 2000, American Society for Biochemistry & Molecular Biology (ASBMB)

    Alessio Peracchi

  55. Functional properties of immobilized pyridoxal 5’-phosphate-dependent enzymes probed by absorption microspectrophotometry

    Article • January 2000, Springer Science + Business Media

    Alessio Peracchi

  56. Identification of the Hammerhead Ribozyme Metal Ion Binding Site Responsible for Rescue of the Deleterious Effect of a Cleavage Site Phosphorothioate†

    Article • Biochemistry, October 1999, American Chemical Society (ACS)

    Alessio Peracchi

  57. Origins of the temperature dependence of hammerhead ribozyme catalysis

    Article • Nucleic Acids Research, July 1999, Oxford University Press (OUP)

    Alessio Peracchi

  58. Structure–function relationships in the hammerhead ribozyme probed by base rescue

    Article • RNA, November 1998, Cold Spring Harbor Laboratory Press

    Alessio Peracchi

  59. A Core Folding Model for Catalysis by the Hammerhead Ribozyme Accounts for Its Extraordinary Sensitivity to Abasic Mutations†

    Article • Biochemistry, October 1998, American Chemical Society (ACS)

    Alessio Peracchi

  60. Involvement of a Specific Metal Ion in the Transition of the Hammerhead Ribozyme to Its Catalytic Conformation

    Article • Journal of Biological Chemistry, October 1997, American Society for Biochemistry & Molecular Biology (ASBMB)

    Alessio Peracchi

  61. Use of intrinsic binding energy for catalysis by an RNA enzyme

    Article • Proceedings of the National Academy of Sciences, August 1997, Proceedings of the National Academy of Sciences

    Alessio Peracchi

  62. Rescue of abasic hammerhead ribozymes by exogenous addition of specific bases.

    Article • Proceedings of the National Academy of Sciences, October 1996, Proceedings of the National Academy of Sciences

    Alessio Peracchi

  63. Time-resolved fluorescence of tryptophan synthase

    Article • Biophysical Chemistry, August 1996, Elsevier

    Alessio Peracchi

  64. Allosteric Regulation of Tryptophan Synthase:  Effects of pH, Temperature, and α-Subunit Ligands on the Equilibrium Distribution of Pyridoxal 5‘-Phosphate−l-Serine Intermediates†

    Article • Biochemistry, January 1996, American Chemical Society (ACS)

    Alessio Peracchi

  65. Monovalent Cations Affect Dynamic and Functional Properties of the Tryptophan Synthase .alpha.2.beta.2 Complex

    Article • Biochemistry, July 1995, American Chemical Society (ACS)

    Alessio Peracchi

  66. Effects of Monovalent Cations on Functional Properties of the Tryptophan Synthase α2β2 Complex in Solution and in the Crystal

    Article • January 1994, Springer Science + Business Media

    Alessio Peracchi

  67. Time course of chemical and structural events in protein crystals measured by microspectrophotometry

    Article • Philosophical Transactions of the Royal Society of London Series A Physical and Engineering Sciences, August 1992, Royal Society Publishing

    Alessio Peracchi

  68. Characterization of tryptophan and coenzyme luminescence in tryptophan synthase from Salmonella typhimurium

    Article • Biochemistry, August 1992, American Chemical Society (ACS)

    Alessio Peracchi

  69. Conformational changes and subunit communication in tryptophan synthase: effect of substrates and substrate analogs

    Article • Biochemistry, August 1992, American Chemical Society (ACS)

    Alessio Peracchi

  70. Allosteric Regulation of Trytophan Synthase: A pKa Change at β-Active Site Induced by α-Subunit

    Article • January 1991, Elsevier

    Alessio Peracchi